To the Editor: High-throughput sequencing is revolutionizing microbial ecology studies. Efforts like the human being Microbiome Projects1 and also the united state National ecological Observatory Network2 are helping us to recognize the function of microbial diversity in habitats in ~ our own bodies and also throughout the planet.

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Figure 1: QIIME analyses the the distal gut microbiotas of conventionally raised and conventionalized mice, gnotobiotic mice colonized with a human being fecal gut microbiota (H-mice), and human adult mono- and also dizygotic twins.


1National institutes of health Human Microbiome task Working team et al. Genome Res. 19, 2317–2323 (2009).

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We thank our collaborators for their beneficial suggestions on features, documentation and also the manuscript, and also our resources agencies for their commitment to open-source software. This job-related was sustained in part by Howard Hughes medical Institute and also grants native the Crohn"s and also Colitis foundation of America, the German academic Exchange Service, the Bill and also Melinda gateways Foundation, the Colorado facility for Biofuels and Biorefining and also the US national Institutes of wellness (DK78669, GM65103, GM8759, HG4872 and its ARRA supplement, HG4866, DK83981 and LM9451).

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J Gregory Caporaso, Justin Kuczynski and Jesse Stombaugh: this authors contributed equally to this work.


Department that Chemistry and Biochemistry, university of Colorado, Boulder, Colorado, USA

J Gregory Caporaso, Jesse Stombaugh, Elizabeth K Costello, Catherine A Lozupone, Daniel McDonald, Meg Pirrung, Jens Reeder, Jeremy Widmann & Rob Knight

Department of Molecular, Cellular and also Developmental Biology, university of Colorado, Boulder, Colorado, USA

Justin Kuczynski, William A Walters & Jesse Zaneveld

Department of Microbiology, university of Pennsylvania, Philadelphia, Pennsylvania, USA

Kyle Bittinger & Frederic D Bushman

Cooperative academy for research study in environmental Sciences and Department that Ecology and Evolutionary Biology, college of Colorado, Boulder, Colorado, USA

Noah Fierer

Department of computer system Science, university of Colorado, Boulder, Colorado, USA

Antonio Gonzalez Peña, Julia K Goodrich & Dan Knights

Center because that Genome Sciences, Washington University college of Medicine, St. Louis, Missouri, USA

Jeffrey i Gordon, Brian D Muegge, Peter J Turnbaugh & Tanya Yatsunenko

Computational dielectrics Laboratory, man Curtin college of clinical Research, The Australian nationwide University, Canberra, Australian resources Territory, Australia.

Gavin A Huttley

Department the Biology, mountain Diego State University, san Diego, California, USA

Scott T Kelley

Department that Microbiology, Cornell University, Ithaca, brand-new York, USA

Jeremy E Koenig & Ruth E Ley

Luca Technologies, Golden, Colorado, USA

Joel R Sevinsky

Howard Hughes clinical Institute, Boulder, Colorado, USA

Rob Knight


Corresponding author

Correspondence to plunder Knight.

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Competing interests

J.R.S. Is one employee that Luca Technologies.